Review



18-ml mini rotofor chamber  (Bio-Rad)


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    Structured Review

    Bio-Rad 18-ml mini rotofor chamber
    Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad <t>Rotofor.</t> The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.
    18 Ml Mini Rotofor Chamber, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/18-ml+mini+rotofor+chamber/pmc00149496-120-19-17?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    18-ml mini rotofor chamber - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Two Nonoverlapping Domains on the Norwalk Virus Open Reading Frame 3 (ORF3) Protein Are Involved in the Formation of the Phosphorylated 35K Protein and in ORF3-Capsid Protein Interactions"

    Article Title: Two Nonoverlapping Domains on the Norwalk Virus Open Reading Frame 3 (ORF3) Protein Are Involved in the Formation of the Phosphorylated 35K Protein and in ORF3-Capsid Protein Interactions

    Journal:

    doi: 10.1128/JVI.77.6.3569-3577.2003

    Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad Rotofor. The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.
    Figure Legend Snippet: Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad Rotofor. The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.

    Techniques Used: Purification, Infection, Recombinant, Staining, Mass Spectrometry



    Similar Products

    18-ml mini rotofor chamber  (Bio-Rad)
    90
    Bio-Rad 18-ml mini rotofor chamber
    Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad <t>Rotofor.</t> The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.
    18 Ml Mini Rotofor Chamber, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/18-ml+mini+rotofor+chamber/pmc00149496-120-19-17?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    18-ml mini rotofor chamber - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad Rotofor. The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.

    Journal:

    Article Title: Two Nonoverlapping Domains on the Norwalk Virus Open Reading Frame 3 (ORF3) Protein Are Involved in the Formation of the Phosphorylated 35K Protein and in ORF3-Capsid Protein Interactions

    doi: 10.1128/JVI.77.6.3569-3577.2003

    Figure Lengend Snippet: Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad Rotofor. The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.

    Article Snippet: The fractions containing proteins with pIs between 4.5 and 4.8 were pooled and refocused by using a Bio-Rad 18-ml mini Rotofor chamber at a constant power of 12 W with 0.5% ampholytes (pH range, 3 to 5) as described above.

    Techniques: Purification, Infection, Recombinant, Staining, Mass Spectrometry